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KMID : 0350519960490020673
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.673 ~ p.687
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The Changes of GFAP-Immunoreactive Astrocytes in Acute and Recovery Phase of Suckling and Adult Rats with Reye's Syndrome Induced by 4-Pentenoic Acid
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Abstract
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We designed this study to evaluate whether any difference of degree of GFAP-immunoreactiviry which is generally accepted as astrocyte-specific marker to brain injury between suckling and adult rats is in existence or not, also to see that the
alterationin the immunoreactivity is reversible in experimental Reye's syndrome induced by the low-dose 4-pentenoic acid (4-PA) in cute and recovery phase.
For this experiment, we selected two age groups of rats, suckling and adult Sprague-Dawley rats, and each animal group was divided into two group; the acute rats which were sacrificed 12 hours after the final injection of 4-PA, the recovery rats
which
were sacrificed 3 weeis after the final injection of 4-PA.
Reye's syndrome was induced by intraperitoneal injection every 4 hour with 25mg/kg body weight of 4-PA for 10 doses, followed by a single dose of 100mg/kg.
Tissue blocks were taken from the cerebrum and cerebellum. Paraffin sections were immunostained with the indirect immunoperoxidase method for light microscopy, and the area percent of GFAP-positive astrocytes was calulated using the image
analysing
system (Vidas 2.0, Kontron, Germany) on the immunostained sections.
@ES The results were as follows:
@EN During the experimental period, no clinical feature of Reye's syndrome was observed I any of rats, but laboratory findings such as hyperammonema, hypoglycemia and the elevated SGOT were similar to those of the Reye's syndrome.
In the acute phase, the GFAP-immunoreactivity and the area percent of GFAP-positive astrocytes in adult groups induced with low-dose 4-PA were significantly increased in cerebral cingulum, cerebellar cortex and medulla, compared with those of
normal
control groups, but not in suckling groups.
In the recovery phase, GFAP-imunoreactiviy and the area percent of GFAP-positive astrocytes in adult groups induced with low-dose 4-PA were significantly decreased, compared with those of acute phase, but there was no difference in the
GFAP-immunoreactivity and the area percent Between acute and recovery phase in suckling groups.
It is suggested that GFAP-immunoreactivity of the astrocytes in adult and even in suckling rats induced by low-dose 4-PA reversibly compensatory changes, and no significance of the immunoreactivity in suckling rats compared with adult rats would
be
related to the sensitivity or vulnerability of immature astrocyte to 4-PA.
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KEYWORD
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